English name: Lysostaphin; Glycyl-glycine Endopeptidase
Other names: Staphylococcus lysase
CAS number: 9011-93-2
Level: BR
Molecular weight: about 27kDa
Specific activity: ≥1200u / mg
Vitality definition: One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 ° C in a 6.0 ml reaction mixture
Enzyme activity measurement method: turbidimetric method for determination of lytic Staphylococcus aureus: 4 ～ 32u / ml
Staphylococcus epidermidis: 8 ～ 64u / ml
Solubility: soluble in 50mmol / L tris-CL
Solution storage method: PH7.5 enzyme solution (PH5 ~ 8) placed at 4 ℃, basically stable in 2-3 days: white or light yellow powder, a metal protease containing zinc, consisting of 246 amino acids. The nucleic acid expressing the gene consists of 1486 nucleotides. The isoelectric point is 10.5-11.0, and the optimal pH range for enzyme activity is 10-11. Hydroxymercury benzoic acid (PCMB), benzene methanesulfonyl chloride (PMSF), dithiothreitol (DTT), etc., at 1 mmol / L, had no significant effect on enzyme activity, and Zn ions showed a certain degree of activation effect ; Ba ions, Mn ions, Ca ions, Mg ions and EDTA under 1mmol / L conditions, show a certain degree of inhibition of staphylococcus lysozyme enzyme Use: Biochemical research. Bacteria that specifically degrade staphylococcus aureus and other cell walls with glycine peptide bond structures can be sterilized by cleaving their cell walls.
Storage: 2 ～ 8 ℃
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