When using a traditional colony counter, the counting pen is connected to the host, the power switch is turned on, the Petri dish is placed on the white light board, the white light is turned on, the counting is touched with the counting pen, and the LED display shows the counted number; the count is available Counting pen temporarily counts the total number on the manuscript paper, turns the power on and off, the LED display automatically resets to zero, and the second count is compared with the previous total number on the manuscript paper. At the same time, according to the regulation of bacterial count inspection, when the number of bacteria growing in a petri dish exceeds 300, the test sample should be diluted and reworked to ensure the accuracy of the count. Therefore, the general colony counter instrument display counts as three digits .
Instructions
When using a traditional colony counter, the counting pen is connected to the host, the power switch is turned on, the Petri dish is placed on the white light board, the white light is turned on, the counting is touched with the counting pen, and the LED display shows the counted number; the count is available Counting pen temporarily counts the total number on the manuscript paper, turns the power on and off, the LED display automatically resets to zero, and the second count is compared with the previous total number on the manuscript paper. The same number can get more accurate results. At the same time, according to the regulation of bacterial count inspection, when the number of bacteria growing in a petri dish exceeds 300, the test sample should be diluted and reworked to ensure the accuracy of the count. .
The traditional colony counting method is time-consuming, labor-intensive, and has a relatively high error rate. It is a fully manual operation method.
With the advancement of technology, colony counting technology is becoming more and more perfect. Mainly reflected in the increasingly higher configuration and more and more complete functions. The counting speed is getting faster and faster, and the accuracy is getting more and more precise. It is embodied in the following aspects:
1. In terms of imaging, the traditional manual colony counter uses a magnifying glass, which can generally be enlarged from 1.7 to 3 times. The colony counter in use now has the function of taking pictures, and the specifications of the CCD are getting higher and higher. The CCD specifications of the instruments on the market are ranging from 3 million to 10 million pixels, and the resolution is from 0.08mm Enhanced to the present 0.01mm, the image clarity is greatly improved. In addition, in response to the needs of the basic microbiology laboratory, it also increases the image feature acquisition function.
2. Image processing function, with image adjustment, image enhancement, image filtering, edge detection, morphological operation, etc., which are not available in traditional colony counters.
3. In terms of statistical functions, it is no longer limited to traditional quantitative statistics. Nowadays, the automatic colony counters appearing on the market have added some intelligent identification technologies, such as full dish colony statistics, area selection statistics, mouse click statistics, sewage area counting, diameter classification statistics, color recognition statistics, etc. The more advanced automatic colony counter also has automatic impurity removal, colony adhesion and segmentation of E. coli technology and so on.
4. The measurement function and the automatic measurement of the bacteriostatic circle are also not available in the original colony counter. More advanced instruments already have drug sensitivity analysis functions. However, this colony counter is mostly used in areas with high data requirements such as microbiology laboratories, and the price is not cheap.
5. In terms of database processing, more traditional colony counters have added data storage, intelligent query, data export, etc. Some can also set the operator's use authority, data modification authority settings, etc., to make the measured data more secure. At the same time, online editing and report printing are also possible.
In short, with the development of science and technology, the colony counter is also constantly improving. Demands can purchase according to their counting focus.
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