Name: Salmonella Culture Medium
Uses: Used for selective separation and cultivation of intestinal pathogens, especially Salmonella and Shigella: Peptone and beef extract powder provide carbon source, nitrogen source, vitamins and minerals; lactose and sucrose are fermentable sugars; Sodium deoxycholate and sodium citrate inhibit Gram-positive bacteria and most of the coliform bacteria and Proteus, but do not affect the growth of Salmonella; sodium thiosulfate and ferric ammonium citrate are used to detect the production of hydrogen sulfide, Make the center of the colony black; neutral red is the ph indicator, the colony of fermented sugar produces acid is red, and the colony of non-fermented sugar is colorless; agar is the coagulant of the medium.
Formula (per liter): peptone 20.0g lactose 10.0g beef paste powder 3.0g sucrose 10.0g sodium deoxycholate 1.0g sodium thiosulfate 2.3g sodium citrate 1.0g ferric ammonium citrate 1.0g neutral red 0.03g agar 16.0g final ph 7.2 ± 0.1 Usage: 1. Weigh 63.3g of this product, add 1 l of distilled water or deionized water, stir and heat to boil to completely dissolve, to be cooled to about 50 ℃, in a sterile environment, pour out Bacterial plate, after solidification, spare. 2. Take one ring of the enrichment solution with the inoculation ring and inoculate it on the plate with a streak. 3. Place the plate in a constant temperature incubator and incubate at 36 ± 1 ℃ for 18-24 hours. 4. Observe the results.
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