Column mollusc DNAOUT products and features This product is a kit specifically for extracting genomic DNA from various mollusc tissues. The principle is based on the cationic detergent CTAB under the appropriate ionic strength conditions, specifically combined with genomic DNA to form a precipitate, and then further purified using silica gel membrane technology. This product has the following characteristics: 1. Suitable for various polysaccharide-rich mollusk animals that are difficult to extract by conventional methods, including snails, shellfish, squid, octopus, and snails. 2. The integrity of the extracted genomic DNA, the longest reachable length is generally 20-50kb. 3. The DNA is pure, OD260 / 280 is generally around 1.9, and the DNA fragment size is around 30-50 Kb, which can be directly used for PCR, enzyme digestion, hybridization, etc. 4. The operation is simple, the whole process takes about 30 minutes. 5. Safe and non-toxic, this kit is non-toxic to human body, and has no corrosive and irritating odor. 6. Low price and good quality, comparable to similar foreign products, but cheaper. Specifications and composition of ingredients 50 times packaging (CAT: 101111-50) solution A 50 mL solution B 50 mL solution C 100 mL centrifugal adsorption column 50 sets of universal column washing solution 50 mL DNA eluent 2.0 10 mL user manual 1 copy Transportation and storage Normal temperature transportation and storage, valid for one year. Bring your own reagent chloroform. Method of use 1. Homogenization method 1: Weigh 0.1-0.3g of mollusk tissue and transfer it to a plastic mortar. After grinding into a powder with liquid nitrogen, transfer it to a clean 1.5 mL plastic centrifuge tube. Note: It is best to avoid the use of silica-containing glass mortar, because DNA will adsorb on the surface, affecting the yield. Add 1 mL of preheated solution A at 65 ° C to the centrifuge tube and mix thoroughly with a pipette tip (if solution A has a precipitate, it needs to be heated and dissolved at 65 ° C, then shaken before use). Go to the third step. 2. Homogenization method 2: Cut 0.1-0.3g of mollusk tissue into small pieces and transfer to 10-15mL plastic tubes (a centrifuge tube for cultivating bacteria), add 1 mL of 65 ℃ preheated solution A, Use a Polytron homogenizer (shear homogenizer) to homogenize for about 1 minute. 3. Hold at 65 ℃ for 5-30 minutes, during which it is best to mix by blowing and mixing 2-3 times. 4. Transfer to a new 1.5 mL centrifuge tube and centrifuge at 12000-15000 g at room temperature for 3 minutes. 5. Transfer no more than 0.75 mL of supernatant to a new centrifuge tube. Sometimes there will be a small amount of fine suspended solids in the supernatant, but it has no effect on subsequent operations and no special treatment is necessary. 6. Add an equal volume of solution B to the supernatant and invert it up and down for 30 seconds to mix thoroughly. The solution will be white and turbid. 7. Place it in an ice bath for 5-10 minutes. 8. Centrifuge at 12000-15000 g for 3 minutes at room temperature, and transfer the supernatant to a new 1.5 mL plastic centrifuge tube. 9. Add 0.2 mL of chloroform (provided by yourself), shake thoroughly on the shaker for 30 seconds to mix. 10. Centrifuge at 12000-15000 g for 3 minutes at room temperature. There will be a white membrane at the interface between the two intersections. Carefully transfer the supernatant to a new 1.5-5mL plastic centrifuge tube. Note: Since the next step is to add 1.5 volumes of solution C, the volume of the new centrifuge tube should be large enough. 11. Add 1.5 volumes of solution C, mix upside down for 30 seconds, and then transfer the mixture to the centrifugal adsorption column in multiple times. 12. After transferring 0.7-0.8 mL of the mixed solution each time, leave it at room temperature for 5-10 minutes. 13. 12000-15000 g at room temperature for 1 minute, discard the penetrating solution. 14. For the samples that need to be loaded on the column multiple times, you can add the remaining samples to the centrifugal adsorption column, and repeat steps 12-14. 15. Add 0.7 mL of universal column washing solution to the spin column, centrifuge at room temperature for 1 minute, and discard the penetrating solution. 16. Add 0.3 mL of universal column washing solution to the centrifugal adsorption column, and centrifuge at 12000-15000 g for half a minute (this step is the second wash). 17. Shake for 1 minute to remove residual liquid. 18. Place the spin column in a new 1.5 mL plastic centrifuge tube and add 30-100 uL of DNA eluent 2.0. 19. Leave at room temperature for 5 minutes and then centrifuge for 1 minute. The bottom of the tube is the mollusc DNA solution, which can be used immediately It can also be stored at -20 ℃ for a long time. 20. You can repeat the previous step once to elute more DNA (the second eluted DNA is generally about 30% of the first).
Training Desk
A training desk is a table designed specifically for activities such as training and meetings. It has characteristics such as durability and ease of movement, which can meet the needs of different groups of people. Training desks can be classified into various categories based on their materials, structure, and other aspects. Below will be a brief introduction to the classification of training desks.
1. Material classification
The materials of the Training Chair mainly include wooden boards, PVC boards, etc. Training desks made of wood usually have strong stability and durability, making them suitable for long-term meetings or training activities. The training table made of PVC board has certain safety performance and is generally more lightweight.
2. Structural classification
The structure of training chairs mainly includes single tables, combination tables, etc. A single chair is the most common training table, and each training table is independent and can be adjusted as needed. Combination refers to the combination of multiple seats together, usually in the form of geometric shapes or rows, suitable for use in large training venues. A stackable table is a training table that can be folded and placed together, saving space and facilitating storage and handling.
Training Table,Tea Table,Coffee Table,Visitor Table
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