Rabbit Anti- GCS antibody manual, FITC conjugated

Catalog Number: RSF-0701R

Quantity size: 0.2ml (2mg / ml, Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium azide. Reconstitute with 0.2ml sterile distilled water.)

Rabbit Anti- GCS antibody manual, FITC conjugatedBackground: GCS (glucosylceramide synthase) may serve as a flippase as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP -glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family.

Specificity: · anti- GCS is a rabbit polycional antibody, FITC conjugated

· Specific for GCS of human, mouse and rat

· Epitope mapping near the amino terminus of GCS of human, mouse and rat origin

· Protein G affinity chromatography purification, purity:> 95%

· Isotype: IgG

· Mol wt: 48kDa

Application: · FCM: 1: 20-100

· Immunohistochemistry: 1: 20-1: 200

· Immunocytochemistry: 1: 20-1: 200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

· Optimal working dilutions must be determined by the end user.

· Protect from light.

Rabbit Anti- GCS antibody manual, FITC conjugatedStorage: Store at –20 ºC for one year. Avoid repeated freeze / thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.