Q: What is the method used in the ELISA kit?
A: The double-antibody sandwich method is currently used, which is a standard approach for detecting target molecules.
Q: How much serum is needed per well?
A: Typically, 10 to 50 microliters of serum are required per well, depending on the protocol and sample concentration.
Q: How much unprocessed sample should I send for testing?
A: You need to send 100 microliters per well for each sample. Make sure it's properly labeled and stored during transport.
Q: How should the samples be stored?
A: For short-term use (within a day), store at 4°C. For samples to be used within a month, store at -20°C. For long-term storage, use -70°C. Avoid repeated freeze-thaw cycles to maintain sample integrity.
Q: What is the purpose of the blank OD value in post-data processing with the mouse acetylcholine ELISA kit?
A: The blank OD value helps subtract background noise from both standards and samples. It's important to ensure consistency by using the same calculation method for all measurements.
Q: Why might the calculated ACH content be negative?
A: This usually indicates that the sample concentration is very low. When the measured value is close to the lowest standard, the linear equation may produce a negative result due to limited sensitivity.
Q: Can tissue specimens be diluted with physiological saline after cutting for the mouse acetylcholine ELISA kit? What is the dilution ratio?
A: Yes, you can dilute the tissue with saline. A 1:3 dilution is commonly recommended, but always follow the kit instructions for best results.
Q: Is there a linear relationship between standard concentrations and absorbance values in the rat 5-HT ELISA kit?
A: Yes, the standard curve typically shows a linear relationship. If your absorbance values are consistently around 0.08, it could be due to low sample concentration, improper dilution, or procedural errors. As long as the values fall within the measurable range, they are acceptable.
Q: In the rat 5-HT ELISA kit, the standard curve is linear, but the sample absorbance is close to the blank. Is this normal?
A: This could happen if the sample has very low 5-HT levels. Other factors like sample preparation, dilution, or instrument settings may also contribute. Ensure proper controls and reagent handling to improve accuracy.
Q: Is the blank control just a well with no sample added?
A: Yes, the blank control typically contains only the sample diluent and no enzyme reagent. It’s used to measure background absorbance.
Q: Is the sample diluent pre-prepared?
A: Yes, the sample diluent is ready to use and should be prepared according to the manufacturer’s instructions.
Q: Can the sample diluent be used directly to dilute serum?
A: Yes, you can add 40 microliters of sample diluent directly to the serum for appropriate dilution.
Q: When using the human copeptin ELISA kit, do I need to work on a static table or can I work outside?
A: It depends on your lab setup. The procedure isn’t highly sensitive to environmental conditions, so it can be performed either way, as long as you maintain good technique and cleanliness.
Q: Is the wash buffer for the human copeptin ELISA kit concentrated or ready-to-use?
A: The wash buffer is concentrated and needs to be diluted before use, as specified in the kit manual.
Q: Can ELISA detect the titer of IgG antibodies in the fourth factor of human platelets?
A: ELISA may not be the best method for quantifying antibody titers in this context. If needed, consider using a fluorescence-based assay for better accuracy.
Q: Does an uneven ELISA plate affect the results?
A: No, slight variations in plate appearance don’t impact the final results. Just make sure all wells are properly filled and incubated uniformly.
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