Q: What is the method used in the ELISA kit?
A: The double-antibody sandwich method is currently used, which is suitable for most standard ELISA procedures.
Q: How much serum is needed per well?
A: Each well requires 10 to 50 microliters of serum, depending on the protocol and sample concentration.
Q: How much unprocessed sample should I send?
A: For each well, you need to provide at least 100 microliters of sample to ensure sufficient volume for testing.
Q: How should the samples be stored?
A: If the sample will be used on the same day, store it at 4°C. For short-term storage (up to a month), keep it at -20°C. For long-term preservation, use -70°C. Avoid repeated freezing and thawing to maintain sample integrity.
Q: What is the purpose of the blank OD value in data processing for the mouse acetylcholine ELISA kit?
A: The blank OD value is subtracted from both the standard and sample readings to eliminate background noise. It's important to maintain consistency across all measurements.
Q: Why might the calculated ACH content come out as a negative number?
A: This usually indicates that the sample concentration is very low. When the sample concentration is near the lowest standard point, the linear equation may produce a negative value due to limited sensitivity.
Q: Can tissue specimens be diluted with physiological saline after cutting for the mouse acetylcholine ELISA kit? What dilution factor is recommended?
A: Yes, you can dilute the tissue with saline. A 1:3 dilution ratio is commonly used, but always follow the manufacturer’s guidelines.
Q: Is there a linear relationship between the standard concentrations and their absorbance values? Why are the absorbance values so low (around 0.08) in most cases? (Rat serotonin, 5-HT ELISA kit)
A: Yes, there should be a linear relationship. Low absorbance values could be due to sample dilution, improper handling, or instrument settings. As long as they fall within the measurable range, the results are still valid.
Q: The standard curve shows a good linear regression, but the sample absorbance is close to the blank value. What could be the reason? (Serotonin, 5-HT ELISA kit)
A: This might happen if the sample has very low 5-HT levels, or due to issues like incorrect dilution, poor control setup, or machine calibration. Double-check your procedure and reagents.
Q: Is the blank control simply an empty well?
A: Not exactly. The blank control typically contains only the sample diluent and no enzyme reagent, to account for background signal.
Q: Is the sample diluent pre-prepared?
A: Yes, the sample diluent is ready to use as provided by the kit.
Q: Can the sample diluent be used directly to dilute serum?
A: Yes, you can add 40 microliters of diluent directly to the serum for proper dilution.
Q: When using human copeptin ELISA kits, do I need to work on a static table or can I work outside?
A: There are no strict requirements. You can work in a clean bench or on a regular lab bench, depending on your lab conditions.
Q: Is the wash buffer for the human copeptin ELISA concentrated or ready-to-use?
A: It is concentrated and needs to be diluted before use, as specified in the kit manual.
Q: Does the ELISA method measure IgG antibody titers in the fourth factor of human platelets?
A: If the ELISA does not detect these antibodies, consider using a fluorescence-based method for better sensitivity.
Q: Does an uneven ELISA plate affect the results?
A: No, minor variations in plate flatness usually don’t impact the results significantly, as long as the procedure is followed correctly.
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