Graduate Student Reagent-Yuzhu

Yuzhu [Pinyin name] Yù Zhú [English name] RHIZOMA POLYGONATI ODORATI [Alias] Wisteria, jade ginseng, tail ginseng, bellflower, small pen tube vegetable, sweet grass root, mangosteen [source] This product is a lily plant Polygonatum odoratum (Mill.) Druce's dry rhizome. In autumn, excavate, remove the fibrous roots, wash, and after sun exposure to softness, knead repeatedly, air dry until no hard core, and dry; or after steaming, knead until translucent, dry. 【Properties】 This product is long cylindrical, slightly flat, few branches, 4 ~ 18cm long, 0.3 ~ 1.6cm in diameter. The surface is yellowish white or light yellowish brown, translucent, with longitudinal wrinkles and slightly raised links, with white dot-like fibrous root marks and disc-shaped stem marks. The quality is hard and brittle or slightly soft, and it is easy to break. It's weak, sweet, and sticky when chewed. [Identification] The cross-section of this product: the epidermal cells are oblate or oblong, and the outer wall is slightly thick and keratinized. Most of the mucous cells are scattered in the parenchyma, with a diameter of 80 to 140 μm, and contain needle crystal bundles of calcium oxalate. The outer tough type of the vascular bundle, rare Zhou wood type, hash. [Determination of content] Preparation of reference substance solution Weigh accurately 60mg of anhydrous glucose dried to constant weight at 105 ° C, place in a 100ml measuring flask, add water to dissolve and dilute to the mark, shake well. 0.6mg). Preparation of standard curve Precisely measure the reference solution 0.0ml, 1.0ml, 1.5ml, 2.0ml, 2.5ml, 3.0ml, put them in 50ml measuring flasks respectively, add water to the mark, shake well. Accurately measure 2ml of the above solutions, put them in a test tube with a stopper, add 1ml of 4% phenol solution, mix well, quickly add 7.0ml of sulfuric acid, shake well, incubate in a 40 ° C water bath for 30 minutes, take it out, and place it in an ice water bath 5 Take it out in minutes, take the first part as a blank, and measure the absorbance at the wavelength of 490nm according to the spectroluminosity method (Appendix VB), with the absorbance as the ordinate and the concentration as the abscissa, and draw a standard curve. The determination method takes 1g of this product coarse powder [at the same time take another product powder to measure moisture (Appendix Ⅸ H first method)], accurately weighed. Place in a round-bottom flask, add 100ml of water, heat and reflux for 1 hour, filter with cotton wool, repeat the extraction once as above, combine the two filtrates, concentrate, move to a 100ml measuring flask, add water to the mark, shake well. Accurately measure 2ml, add 10ml of ethanol, stir, centrifuge, dissolve the precipitate and dissolve in water, place in a 50ml measuring flask, and dilute to the mark. Accurately measure 2ml, according to the method under the preparation of the standard curve, add 4% phenol Starting from 1ml of solution, measure the absorbance according to the law, read the polysaccharide content (μg) of Yuzhu polysaccharide in the test solution from the standard curve, and calculate it. This product is calculated on the basis of dry products. The polysaccharides contained in Polygonatum vulgaris, calculated as glucose (C6H12O6), shall not be less than 6.0%. [Processing] Remove impurities, wash, moisten, cut thick pieces or segments, and dry. 【Sexual flavor】 Sweet, slightly cold. 【Missing】 Return to the lung and stomach. 【Functions】 nourishing yin and moisturizing, relieving thirst. It is used for lung and stomach yin wounds, hot cough, dry thirst and thirst, internal heat and thirst. 【Usage and dosage】 6 ~ 12g. [Storage] Store in a ventilated and dry place, prevent mildew and moth.

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