The kit employs a double-antibody sandwich immunoassay to quantify the concentration of human osteopontin (OPN) in a given sample. The process begins with a microtiter plate pre-coated with a purified monoclonal antibody specific to OPN, forming a solid-phase capture antibody. After incubation, the sample is added, allowing OPN to bind to the immobilized antibody. A horseradish peroxidase (HRP)-labeled secondary antibody is then introduced, which binds to the captured OPN, forming a stable antibody-antigen-enzyme complex. Following a thorough wash to remove unbound components, a substrate solution containing TMB (3,3',5,5'-tetramethylbenzidine) is added. Under the catalytic action of HRP, TMB is oxidized to a blue product, which is then stopped by adding an acidic solution, resulting in a yellow color. The intensity of the yellow color is directly proportional to the amount of OPN present in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, and the OPN concentration is determined by comparing the OD value to a standard curve generated from known concentrations of OPN. This method ensures high specificity, sensitivity, and reproducibility, making it ideal for clinical and research applications.
As Baby grows from infant to toddler, what they need from their car seats will change. Your child`s age, weight & height determine the right seat for their safety.
Car Seat Safety Tips
1. As children grow, the type of car seat they use will change. It`s important to check your child`s fit and the car seat installation regularly.
2. Before installing your car seat , it's important to make sure it fits your child. Selection of car seats or boosters should be based on your child's age, weight, height and developmental level.
Car Seats,Toddler Car Seat,Baby Car Seat,Child Car Seat
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