Instructions for use of mouse substance P receptor (SP-R) elisa kit
Elisa kit specifications: 48-well configuration / 96-well configuration
Standard diluent: 1.5ml × 1 bottle
Enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96)
ã€Mouse P receptor (SP-R) elisa kit】 This reagent is for research use only
Calculation:
Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; then multiply by the dilution factor; Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample.
Kit composition:
Sealing film: 2 pieces (48) / 2 pieces (96)
Instructions: 1 copy
Sealed bag: 1
Standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle Store at 2-8 ℃
Enzyme-coated plate: 1 × 48 1 × 96 2-8 ℃
Sample diluent: 3ml × 1 bottle 6ml × 1 bottle Store at 2-8 ℃
Developer A solution: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Developer B: 3ml × 1 bottle 6 ml × 1 bottle Store at 2-8 ℃
Stop solution: 3ml × 1 bottle 6ml × 1 bottle Store at 2-8 ℃
Concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle Store at 2-8 ℃
Storage conditions and validity period:
Kit storage: 2-8 ℃ | Validity: 6 months
[Mouse Substance P Receptor (SP-R) elisa kit] Sample processing and requirements:
1. Serum: room temperature blood coagulates naturally for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage.
2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
3. Urine: collected in a sterile tube and centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, centrifuge again. Pleural and ascites, cerebrospinal fluid reference implementation.
4. Cell culture supernatant: When detecting secreted components, collect with a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting the components inside the cells, dilute the cell suspension with PBS (PH7.2-7.4), and the cell concentration will reach about 1 million / ml. Through repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
5. Organize the specimen: after cutting the specimen, weigh it. Add a certain amount of PBS, PH7.4. Quickly freeze and save with liquid nitrogen for later use. After the specimen melts, it still maintains a temperature of 2-8 ° C. Add a certain amount of PBS (PH7.4) and homogenize the specimen with a manual or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. After aliquoting, a portion is to be tested, and the rest is frozen for future use.
6. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided.
7. The sample containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
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