Shanghai Jinma Rat Insulin (INS) ELISA Kit Latest Instructions

Experimental principle The kit uses a double antibody sandwich method to determine the level of insulin (INS) in the specimen. The microplate was coated with purified rat insulin (INS) antibody to prepare a solid phase antibody, and insulin (INS) was sequentially added to the microcapsule of the coated monoclonal antibody, and then combined with HRP-labeled insulin (INS) antibody to form The antibody-antigen-enzyme-labeled antibody complex was thoroughly washed and added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with insulin (INS) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat insulin (INS) in the sample was calculated from a standard curve.

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