At 15 o'clock yesterday afternoon, my manager He received a call from Manager Li of the technical department. Manager Li said: "I have summarized this month's kit customer consultation and found that there are several points that are often ignored by customers. I put The key content is sorted out and sent to your mailbox. Go inside and check it out. Sharing it can help the customers' experiments.†The following is the latest content brought by Shanghai Hengyuan Technology Manager Li, August technical summary Elisa Test kit detection points:
1. Read the instructions carefully to confirm that the kit is within the validity period.
2. According to the instructions, all reagents are complete, as well as all the extra items required for the experiment, such as test tubes, tips, pipettes, centrifuges, etc.
3. Specimen preparation should be standardized. The volume of each specimen should be prepared (stored) according to the amount of 2-3 or more duplicates. Those who are unable to experiment in the short term, pay attention to cryopreservation.
4. Prepare the required reagents by equilibrating the reagents used to room temperature according to the instructions.
5. When selecting antibodies, it is best to choose a monoclonal antibody and a polyclonal antibody for pairing; if two monoclonal antibodies are selected, antibodies with different epitopes must be identified; it is best to select antibody pairs from the same company.
6. The linear range of the standard curve of the antigen to be tested can be obtained by performing a 2-fold serial dilution of the standard from 2000 pg/ml to 15 pg/ml. Sensitivity can be increased to a certain extent using standard Elsia protocols, amplification kits, reagents of the third type, or changes to the enzyme substrate system.
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